FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002300336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810768
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG145080.24967439760114327No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT125190.2154448430913091No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA117970.20301963527024314No Hit
GTCGGTGACAAGAAGAGAGGTGGCGTGACCTGTGGATACTGAGGAAGCGT112760.19405352270130213No Hit
GGCCTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG111550.1919711817783811No Hit
GTCGGTGACATGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC94810.16316259743978764No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGAGGTGG90680.15605510321527208No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC90330.15545277319624531No Hit
GGCGTGACCTGTGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGG84640.14566060802978195No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA84250.144989440294295No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA83310.1433717539574803No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG82760.14242523535615256No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT75240.1294837446616351No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC74240.1277628017501301No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC72180.12421765935242983No Hit
GTCGGTGACAAGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC71940.12380463305366864No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC68610.11807389315835705No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC68380.11767807628871088No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC66310.11411572446189558No Hit
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC66280.11406409617455042No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG66180.11389200188339993No Hit
GGCGTGACCTGTGGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGGGTGG66160.11385758302516982No Hit
GCTGGGAGTACTGTGTGAGGGGGTCTCAGAGGTGGTTATCAAAGAAATGA64630.1112245403705672No Hit
GGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGTG63550.10936592202614182No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC62980.10838498456658396No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG62290.10719753395764553No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA61830.10640590021835325No Hit
GGCTGGTGACAGGAAGAGGGGTGGCCTGTCCTGTGGATGCTGAGGAAGTG61800.10635427193100809No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT61490.10582077962844154No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA61120.1051840307511847No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC59710.10275750124596267No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT59280.10201749579401552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)