FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5810768
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC165780.2852979158692964No Hit
CCACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACC156820.26987826738221177No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC133850.23034820870494227No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG109260.18803022251103468No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC100560.17305801918094132No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC95730.16474586491837223No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA90300.15540114490890017No Hit
CTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACT88080.1515806516453591No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC87550.15066855190226147No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC83170.14313082194986962No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC82930.14271779565110843No Hit
CCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC80540.13860474209261153No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT79740.1372279877634075No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC78870.1357307674303982No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCATCTTCCTGTCACC74340.12793489604128058No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC73930.12722930944756355No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC72680.12507813080818234No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC72440.12466510450942113No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC72220.12428649706889003No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC70810.121859967563668No Hit
TCCACAGGTCACGCCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATC69370.11938180977110083No Hit
CGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACCG68910.11859017603180852No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC67470.11611201823924136No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC65980.11354781330109892No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT61460.10576915134109638No Hit
GGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGG60170.10354913498525496No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT59890.10306727097003357No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACCCCTCTTCCTGTCACT59290.10203470522313057No Hit
AGCCCTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACCGA58360.10043422831543093No Hit
CCACAGGTCACGCCACCCCTCTTCATGTCACCGACGCTTCCTCAGTATCC58280.10029655288251055No Hit
CCGACACTTCCTCAGCATCCACAGGACAGGCCACCCCTCTTCCTGTCACC58120.10002120201666974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)