FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997913
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN473531.579532161206813No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT119760.399477903461508No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA115010.3836335477380431No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC105020.3503103659112189No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG68460.2283588616480865No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC65470.2183852566768949No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT64900.21648393399007912No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG64380.21474939399508927No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT63520.21188073169568297No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG62630.2089119997811811No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC57220.19086611252561364No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA54720.18252697793431633No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC54490.18175977755191694No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC53910.17982509832673596No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC52810.17615587910656513No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC52350.17462147834176642No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT51490.17175281604236015No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT51340.1712524679668823No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG50090.16708290067123363No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC49710.16581535221335644No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC48940.16324689875923684No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC47920.15984453184598751No Hit
GCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTG45920.15317322417294965No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA45420.1515053972546902No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG44830.149537361491144No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC44430.14820309995653644No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC44090.14706897765212No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA44090.14706897765212No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC43840.14623506419299026No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC43190.14406688919925295No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC41620.1388299126759182No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT41180.1373622249878499No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG41130.13719544229602393No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT39870.13299251846201007No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT39680.13235874423307148No Hit
GCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT37310.1244532446405216No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA37040.12355261810466148No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA36810.12278541772226212No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC36750.12258527849207097No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT35880.1196832596542995No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA35650.11891605927190016No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC34440.11487991812971224No Hit
CCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCG34110.113779152363661No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC33350.11124405544790658No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC33110.11044349852714205No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG33030.11017664622022053No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC32660.10894245430070854No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC32410.10810854084157878No Hit
GCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTG32370.10797511468811803No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA31240.10420582585285162No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCT30860.10293827739497445No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT30060.10026975432575928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)