FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327067
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA188420.5663246336788529No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG183010.5500640654366143No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC102610.30840977954456583No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA95910.28827192238689514No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA90140.270929320028722No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT86960.2613713520046335No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG86880.2611308999788703No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG86520.26004886586293574No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC84180.25301564410936117No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG83980.25241451404495313No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT82070.24667372192985595No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC78150.23489157266745755No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC75000.2254237741530303No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA74690.22449202255319778No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC72940.21923213448962706No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC68690.2064581206209553No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT62400.1875525800953212No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT57560.17300523253664563No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG57330.17231393296257633No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG55340.16633268882171595No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT53260.16008093615187188No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC51510.1548210480883012No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC50360.15136455021795475No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA49480.14871957793455917No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT48970.14718669627031858No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA48590.1460445491479432No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT48040.144391441470821No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA47530.14285855980658038No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT45160.13573516854334464No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG45150.13570511204012423No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC45120.13561494253046302No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTG45030.13534443400147939No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC44500.13375143933079797No Hit
GTCCTTTTTAATTCCTGCAATACATTCAGACAGGGACAGACAAAATAAGC44220.13290985724062665No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG42970.12915279433807617No Hit
ATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAGG40590.12199934657162No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA39830.11971505232686928No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC39780.11956476981076726No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG37520.11277200008295596No Hit
CGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTT37220.11187030498634382No Hit
GCCCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAG37140.1116298529605806No Hit
GCTTGGTTGAAAAGATGTAATCGATAATGTCAGCCCGCTCTGTTTTCCCT36720.11036747982532363No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC36070.10841380711599737No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA35780.10754216852260565No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG34430.10348454058785111No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT34000.10219211094937372No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC33590.10095979431733715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)