FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559115
Sequences flagged as poor quality0
Sequence length126
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA189360.5320423757029487No Hit
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC136990.3848990549616969No Hit
GTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT100580.2825983425654973No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG99980.2809125302216984No Hit
CTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGAT92100.25877219477313884No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTACTGTCGA63060.17717887733326965No Hit
CCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCC61890.1738915432628617No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG60720.17060420919245375No Hit
CCTCCATTCACCAGTCTCAGGGCCAAAGTGGATTCAGATCCTGCTGTTGA58950.16563106277824685No Hit
CCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCAC57660.1620065662390791No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG56910.15989930080933043No Hit
CTGCAAACCACATTGGCATCATTGGTGTCCCAGTAGTCATCACACACGGT55890.1570334198248722No Hit
GTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAG52460.14739619259282155No Hit
GGCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTC51010.1433221460953074No Hit
CCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC49610.13938858395977652No Hit
CCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCA49180.1381804184467206No Hit
GTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATG48060.1350335687382959No Hit
GTCGGCAATGTGTCTGGACTGGGCGTCGACCAGGAGTGGGCAGCTGAGCA48050.13500547186589923No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC47790.13427495318358637No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG45650.12826222249070343No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC44730.12567731023021173No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG44110.12393530414161948No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT42640.11980506389931206No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT41440.11643343921171415No Hit
GTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGC41430.11640534233931751No Hit
GCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCG40860.1148038206127085No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT39940.11221890835221678No Hit
CTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCACC38180.10727385881040652No Hit
GGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGAT37530.10544756210462433No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA37420.10513849650826118No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT36930.10376174976082536No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA36790.10336839354727229No Hit
CTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCCA36110.10145780622430015No Hit
CTGCTGTTGATGCACGAGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC36040.1012611281175236No Hit
GCGTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCA36020.1012049343727303No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)