FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3559115
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN415421.1672002731015996No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG74090.20816972758677366No Hit
CATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAA66220.18605748901061078No Hit
GGCAGCTGGGCTGTGGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTT53010.14894152057463725No Hit
CAATGATGCCAATGTGGTTTGCAGGCAGCTGGGCTGTGGCTGGGCCACGT51860.14571038024902258No Hit
CCCAGCCCAGACACTTGGCCAACCTCACATGCATCAACAGCAGGATCTGA51780.14548560526984938No Hit
CATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGC51160.14374359918125715No Hit
GGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAG50440.1417206243686984No Hit
GTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCC50340.1414396556447319No Hit
CAGGACATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCC45880.12890845055582636No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA45090.1266887976364911No Hit
CCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATG44360.1246377259515357No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC43270.12157516686030095No Hit
GACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATGAGTCCTATCTG42460.11929932019617237No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT42290.11882167336542933No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT42030.11809115468311646No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT41940.11783828283154661No Hit
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC39870.11202223024544024No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC39250.11028022415684798No Hit
CATGAAGACGCTGGTGTCATCTGCTCAGCTTCCCAGTCCCAGCCGACACC37290.10477323716710475No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC36980.10390223412280863No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)