FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002300383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029631
Sequences flagged as poor quality0
Sequence length126
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA34270.33283768651099277No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT28310.2749528714656027No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC22600.21949611074258643No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC21750.2112407260465157No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT19760.1919134136404207No Hit
CTTCTTCAATCAGCACCACTGAGACCACATCCCACAGTACTCCCAGCTTC19610.19045658104699645No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA18610.18074436375750147No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG17410.1690897030101075No Hit
TCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTT15910.15452137707586505No Hit
CTTCTTCGATCACCACCACCGAGACCACATCCCACAGTACTCCCAGCTTC15830.15374439969270545No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC15410.14966526843111758No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC14720.14296383850136601No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT13780.13383435424924073No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT13590.1319890329642367No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC13350.12965810081475793No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA12970.1259674582447498No Hit
CCACAACAGCCACTACGACTGAGTCCACTGGATCCACGGCCACCCCGTCC12400.12043149438973769No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT12130.11780919572157403No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC12100.1175178292028892No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC11730.11392430880577606No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT11580.11246747621235181No Hit
GTTCAAGATGTGCTTCAACTATGAAATCCGTGTGTTCTGCTGCAACTACG11120.10799985625918411No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT10870.10557180193681037No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC10830.10518331324523057No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC10520.10217252588548714No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC10410.10110418198364268No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC10410.10110418198364268No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC10400.10100705981074773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)