FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3239505
Sequences flagged as poor quality0
Sequence length126
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA81850.25266205793786395No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC63190.19506066513248166No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT57350.17703321958138665No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA57010.17598367651848046No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC55360.17089030577202383No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC53040.16372871781336965No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA50840.15693755681809413No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC48700.15033160930450795No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT48680.15026987147727816No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC45850.14153396892426467No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG45310.13986704758906068No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC43690.1348662835834487No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC43640.13471193901537426No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC41300.12748861322949032No Hit
TCCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATC38870.11998746722107236No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC38700.11946269568961926No Hit
CCACAGGTCAGGCCATCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCC37890.11696231368681326No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACCCCTCTTCCTGTCACC37430.11554234366052839No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC36600.11298022383049262No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA36300.11205415642204597No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC35210.10868944483802309No Hit
CCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN33930.10473822389531734No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG32700.10094134752068602No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC32440.10013875576669892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)