FastQCFastQC Report
Fri 31 May 2024
EGAF00002300392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165260
Total Bases383.1 Mbp
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG162060.511995855000853No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA128590.40625414657879605No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG120280.3800003791157756No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT118330.37383974776163725No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC117430.37099637944434266No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG114350.36126574120293437No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA113750.35937016232473795No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC107730.3403511875801672No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT85180.26910901474128507No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA77000.24326595603520723No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA75220.2376424053632245No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG73500.23220841257906144No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT70950.22415220234672664No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG69050.21814953589910463No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG66160.20901916430245857No Hit
GAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGT64180.20276375400441038No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC63930.20197392947182854No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG62840.19853029450977172No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG60630.19154824564174822No Hit
CCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTG60550.19129550179132204No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT58920.18614584583888844No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC57840.18273380385813487No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC57580.18191238634424975No Hit
GGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAG56830.17954291274650422No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG56260.17774211281221763No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC55820.1763520216348736No Hit
GGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTT54920.17350865331757895No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT54660.17268723580369386No Hit
GGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCAT54000.17060209903767778No Hit
TCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC52090.16456783960875251No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC50080.1582176503667945No Hit
GTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGC48990.15477401540473767No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC47690.1506669278353121No Hit
GGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAA46510.14693895604152582No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC46170.1458647946772145No Hit
CTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTG44280.13989372121089577No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG43990.13897752475310085No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG43880.13863000195876485No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT43460.13730309674402735No Hit
GCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATT41820.13212184781029046No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA40550.12810953918477472No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT39900.12605599540006193No Hit
GCTGGGAGTACTGTGTGAGGGGGTCTCAGAGGTGGTTATCAAAGAAATGA39280.12409723055925896No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT39180.1237813007462262No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG39000.12321262708276728No Hit
AGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGT38730.12235961658757889No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC38700.12226483764366909No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA37620.11885279566291553No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG37500.11847367988727625No Hit
TGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAG36750.11610420628953072No Hit
GCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCC34920.11032269071103165No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC34800.10994357493539235No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA33860.10697383469288463No Hit
CGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCC33750.10662631189854863No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA33740.10659471891724534No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA33730.10656312593594207No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC33710.10649993997333553No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTT33100.10457276811383584No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT32600.10299311904867214No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT32130.10150824892741829No Hit
GGACTCTTTCTGGATGTTGTAGTCAGACAGGGTGCGTCCATCTTCCAGCT32020.10116072613308227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)