FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1660506
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC49980.3009925890060018No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC44210.2662441448570496No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC42740.2573914216509907No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA39230.23625328664876852No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT37320.22475076874157637No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC35560.21415158993704328No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC35350.2128869151933206No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC35290.21252557955225695No Hit
CATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA35070.21120068220169033No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT33940.20439552762832533No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG33420.20126395207244055No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC32950.19843348955077547No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC30160.1816313822413168No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAAGTG29680.17874069711280777No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG29560.17801802583068052No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT29410.17711468672802147No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG29110.17530800852270334No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT28170.16964708347937316No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC27910.16808129570143077No Hit
CATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAG27530.16579283664136113No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC26730.1609750280938461No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA26290.15832523339271282No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT25840.1556152160847356No Hit
CAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTC25550.1538687604862614No Hit
CTTTGCCGGAAAGCAGCTGGAAGATGGGCGCACCCTGTCTGACTACAACA25540.15380853787941748No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC25010.15061673971668876No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG23720.14284802343382078No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT23650.1424264651859132No Hit
ATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGC23600.14212535215169353No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA23360.140680009587439No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGC23170.1395357800574042No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC22830.1374882114247103No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG22170.1335135193730104No Hit
CTCCTGACCAGCAGAGGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGA22160.13345329676616646No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA21800.1312852829197847No Hit
GCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGGAGGAAA20910.12592547091067421No Hit
CATCACTCTCGAGGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAA20530.12363701185060458No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA20430.1230347857821652No Hit
CTTTGCCGGAAAACAGCTGGAAGATGGTCGTACCCTGTCTGACTACAACA20400.12285411796163338No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT20230.12183033364528642No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT19920.11996343283312437No Hit
CCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATCTTTGCTG19580.11791586420043047No Hit
GCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTG19380.1167114120635517No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT19300.11622963120880021No Hit
CCTGTCCCTGGCCGACAAGGATGTGACTGCCAAAGACAGCAAGTTCAAAA19100.11502517907192145No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT18850.113519613900823No Hit
CCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGT18830.11339916868713512No Hit
CTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAGAG18740.1128571652255397No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC18580.1118936035160367No Hit
CGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCAC18110.1090631409943716No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT17990.10834046971224434No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC17290.10412488723316868No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC17250.10388399680579294No Hit
CAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCAC17210.10364310637841717No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA17180.10346243855788537No Hit
ATTGAGAATGTCAAGGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGA17090.10292043509628993No Hit
TGCAGATCTTCGTGAAGACCCTGACTGGTAAGACCATCACTCTCGAGGTG17000.10237843163469447No Hit
CTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTCCGTCTCAGAG16930.10195687338678693No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC16670.10039108560884454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)