FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002300396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985475
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG84350.42483536685176093No Hit
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC80620.40604893035671563No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT61780.31115979803321625No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG59090.2976114028129289No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT53100.2674422997015828No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT52730.2655787657865246No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA51430.25903121419307723No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG49740.2505193971215956No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA49610.24986464196225083No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT46590.23465417595285765No Hit
CTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGAT41900.21103262443495888No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA39110.19698057139979097No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA37840.19058411715080775No Hit
CTGCAAACCACATTGGCATCATTGGTGTCCCAGTAGTCATCACACACGGT36690.18479205227968118No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT34380.17315755675594No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA31920.16076757450987797No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG29860.15039222352333825No Hit
CTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGA29290.147521373978519No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT28740.14475125599667585No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT28720.14465052443369975No Hit
CTCGGAAGGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCC28540.1437439403669147No Hit
CTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGT27130.13664236517709868No Hit
CTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACGAA26860.13528248907692114No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG26540.1336707840693033No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC26090.13140432390234075No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC25480.12833201123156926No Hit
GCTGGGAGTACTGTGTGAGGGGGTCTCAGAGGTGGTTATCAAAGAAATGA24460.12319470151978745No Hit
GGGGTCTCAGAGGTGGTTATCAAAGAAATGAAGCTGGGAGTACTGTGTGA24430.1230436041753233No Hit
GGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGC24190.12183482541960992No Hit
CTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTA23320.11745300243014897No Hit
GCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATG22760.11463251866681776No Hit
CACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGCTCCACAGATAGGAC22730.11448142132235359No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC22710.11438068975937747No Hit
CGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCAAACCGGGCATT21860.11009959833289264No Hit
AGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGA21660.1090922827031315No Hit
CTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAAT21630.10894118535866731No Hit
CCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCCA21520.10838716176229869No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC21420.10788350394741814No Hit
CTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCG21270.10712801722509728No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGGTCCTGAGCAGAT20910.10531484909152722No Hit
CCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGGA20890.1052141175285511No Hit
CTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCACC20460.10304838892456465No Hit
CCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCTGCTGAGGGTGG20450.1029980231430766No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG20210.10178924438736324No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT19920.10032863672420958No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)