FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338606
Sequences flagged as poor quality0
Sequence length101-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG202540.6066603846036339No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA201460.6034255015416614No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG143200.4289215319208077No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA143140.42874181619514257No Hit
GCTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGT128780.385729852519285No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC128240.38411241098829874No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT126390.37857117611362345No Hit
CCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCA114610.3432869886413671No Hit
CCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGT99960.29940639895812804No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC98780.2958719896867136No Hit
CCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAA97900.2932361590436248No Hit
CCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGG94030.28164449473822306No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT89460.26795614696672804No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC87020.2606477074563456No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA83870.25121263185892556No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC82400.24680959658012958No Hit
CCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTT80660.2415978405358404No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGG78520.23518797965378363No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC77430.23192314397086689No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC76290.22850854518322916No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA71530.21425109761379452No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC66550.19933469238358764No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG64400.1928948788805867No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG63950.19154701093809812No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG63570.19040881134221888No Hit
AGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAGT62190.1862753496519206No Hit
TCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT61090.18298056134805962No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC61080.18295060872711547No Hit
CTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAAC58380.17486340107218404No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG57220.17138889704265792No Hit
AGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAA56890.17040046055149963No Hit
CCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTC56110.16806415611785278No Hit
CATCCATGTCAACAGCCAAGCCAACACGCTGTTGCTCCAATACATAAAAG55740.16695590914291772No Hit
AGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAA55250.16548823071665242No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG52600.15755078616644191No Hit
TCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTC52290.15662225491717202No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT50830.1522491722593202No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT50640.15168007246138057No Hit
TCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAA49730.14895438395545926No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT49670.1487746682297941No Hit
TCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC49220.14742680028730554No Hit
GTGGGTTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTC47640.14269428617812346No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA46950.1406275553329743No Hit
GGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAG45160.1352660361839642No Hit
CCCTGCTGAGCCTGGGGCATGGTGGGTTTTGATCTTCTTCCCCTTCTTTT44450.13313940009692668No Hit
CCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAA44150.13224082146860097No Hit
GTAGGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAAT43710.1309229061470566No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC43670.13080309566327983No Hit
CTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCT43400.12999437489778667No Hit
CAGGGTCTTCACGAAGATCTGCATCCCACCTCTGAGACGGAGCACCAGGT43270.1296049908255122No Hit
CTCCCTGCTGAGCCTGGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTT42750.1280474545364143No Hit
CTTCCATAGGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGT42350.1268493496986467No Hit
GGTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCC42250.12654982348920477No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC42090.12607058155409773No Hit
CAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTATCCAGTGAG41710.1249323819582185No Hit
GTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCCTGCTGAGCC41580.124542997885944No Hit
CCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGC40540.12142792530774821No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG39960.11969067329298516No Hit
CCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT39930.1196008154301526No Hit
CACTCGGCTCCACTTCGAGAGTGATGGTCTTACCAGTCAGGGTCTTCACG39270.11762394244783601No Hit
CCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTG38700.11591664305401715No Hit
CTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCCTGG38350.11486830132097049No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT38270.11462868035341696No Hit
CACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAAC38140.11423929628114249No Hit
GCTCAGTTACCTGGGGCATGGTGGGCCTTGGTCTTCTTCCTCTTCTTGGT38050.11396972269264477No Hit
CCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAA37990.11379000696697962No Hit
CCCCCTTCTTTTCAATTTCTCCCACGTCAAGAGAAAAGCCAACATGTTTT37970.11373010172509125No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT37280.1116633708799421No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT36670.1098362610023465No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTG36120.10818886685041601No Hit
GGGGCATGATGGGTCTTGGTCTTCTTCCACTTCTTGGTACTTTTCAATTT35500.10633180435187621No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG35480.10627189910998783No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA35310.10576270455393659No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG35180.10537332048166209No Hit
GTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCCTGCTGAGCC34530.10342640012028972No Hit
GGGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAAC33810.10126981141230801No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)