FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338606
Sequences flagged as poor quality0
Sequence length101-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC182410.5463657586429785No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC149120.4466534835197684No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC133340.3993882476698358No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT108880.32612413684034597No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC97460.2919182437220804No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA85320.2555557618958332No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT83830.25109282137514877No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC79960.2395011570697471No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC78050.2337802064694067No Hit
AGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTT76500.22913755022305718No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT75730.2268311984103545No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC74600.22344655224366097No Hit
TGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATTGG72330.2166473072893297No Hit
GAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCC72110.21598834962855756No Hit
AGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGC71360.21374190305774327No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA71320.2136220925739665No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC66090.19795687182015487No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT65430.1959799988378383No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC63870.19130738997054458No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA63700.19079819541449336No Hit
GTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCCC63240.18942037485106059No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC62420.18696425993363697No Hit
AGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA62140.18612558654719963No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG61860.18528691316076232No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA61020.1827708930014503No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC59670.1787272891739846No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC59220.17737942123149603No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG58620.17558226397484458No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA58570.17543250087012363No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG57050.1708797024866067No Hit
GATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAA55110.16506889402343375No Hit
CCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGG54300.16264273172695431No Hit
CGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAAGGCCCAC53600.16054604826086097No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA53180.15928803818120496No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAG52760.15803002810154898No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA50150.15021239403511527No Hit
GAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC49870.14937372064867793No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT49810.14919400492301277No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC48630.1456595956515983No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT48000.1437725805321143No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC47740.14299381238756534No Hit
CACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGAC47730.14296385976662115No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTACATATT47390.14194547065451868No Hit
AGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGA47010.14080727105863944No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG43930.13158186380782877No Hit
GCTGGATGAGAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT43720.13095285876800078No Hit
AAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCA43240.12951513296267964No Hit
GTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCCC42600.12759816522225145No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC42520.12735854425469792No Hit
AGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCC42370.12690925494053507No Hit
CATTGGAGGAACAGTACCTTGGCTTGGCTCTTGACGTGGACAGAATTAAA42070.12601067631220936No Hit
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG41870.12541162389332552No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC41560.12448309264405563No Hit
GGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCAT41220.12346470353195316No Hit
CTCTTGACGTGGACAGAATTAAAAAGGACCAAGAAGAGGAAGAAGACCAA41030.12289560373401354No Hit
AATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGA41020.12286565111306934No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA40340.1208288728888644No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC39950.11966072067204096No Hit
AGAAGACCAAGGCCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGAGG37750.11307114406431905No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG37750.11307114406431905No Hit
AGACAGTGGACTGTTATGGGTGTAGGTGAATTGGCTTATTTTGTCTGTCC37750.11307114406431905No Hit
CACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGA37120.11118412894483506No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC36950.11067493438878383No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG36610.10965654527668135No Hit
CTCACTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTG36150.10827872471324859No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT36030.10791929326191829No Hit
GATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAG34920.10459455233711315No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT34870.1044447892323922No Hit
ATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGC34810.10426507350672705No Hit
CTTGGCTGTTGACATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAG34620.10369597370878743No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC34610.10366602108784326No Hit
GGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGC34530.10342640012028972No Hit
GATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAG34300.10273748983857334No Hit
GCCTGAGATCCCTGTGTGGGGATTAGACAGTGGACTGTTATGGGTGTAGG33680.10088042734003354No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG33470.10025142230020553No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)