FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789064
Sequences flagged as poor quality0
Sequence length126
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC387440.3957886065511473No Hit
GCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT255300.2608012369721967No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA220000.22474058806848132No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG188980.19305216515082546No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC182600.18653468809683948No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT181590.18550292448797967No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG171570.1752670122495879No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGT148740.15194506849684505No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT147270.15044339274929655No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT135780.13870580476335634No Hit
TCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG119610.12218737154032296No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG111400.11380046141285825No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT107570.10988793208421153No Hit
AGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT106040.108324963449008No Hit
GGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC100550.1027166642285718No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)