FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002300469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4476245
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC148460.33166191752238766No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC114050.2547894496391507No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC91520.20445708400679585No Hit
GTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTAT88070.19674973108040333No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA86240.1926614830064038No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT85640.1913210738018138No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGC83690.18696474388689627No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT80230.1792350508070939No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT79210.17695635515929087No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC78950.1763755111706352No Hit
CCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAG69850.15604597156768676No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT69250.15470556236309674No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC68900.15392365699375257No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG64870.1449205751695897No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC62420.13944723758418048No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG58530.13075691790775526No Hit
CAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAAC55770.12459103556664124No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA53630.11981024273693688No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT52940.11826877215165836No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAGGCTC52290.11681666218001918No Hit
CCAAGACCCATCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG52220.11666028110615037No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT51790.11569965450952752No Hit
CCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGC51520.11509647036746201No Hit
GGTGAATTGGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAATT50920.113756061162872No Hit
CCTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAGACC50910.11373372100946218No Hit
CTGGATAGATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTT50810.11351031947536383No Hit
CCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACCTCACACAGTAC50480.11277309441283932No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA48670.10872952664565946No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC48300.10790294096949564No Hit
TCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAG47840.10687529391264329No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG46830.10461893841825012No Hit
CCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGGGCCTGAAGTCTTGCA46670.10426149596369277No Hit
GTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTAT45900.10254130415113559No Hit
ATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCCTAT45330.1012679154067751No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)