FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410050
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA67000.2780025310678202No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG62260.25833488931764903No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC54370.22559697931578182No Hit
CTCTGCTCCTGTCCAGAAGGCACCACCACAGCCGCCCACTTCTGGAAGGT47860.19858509159561005No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG42630.17688429700628616No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG40960.16995498018713304No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC40250.16700898321611585No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA36890.15306736374764007No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA34340.14248667040102903No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT32740.13584780398746915No Hit
CCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAG31210.1294993879795025No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT28380.11775689301051846No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCCCTTTCTCATCCAGCAGCTCCC28370.1177154000954337No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG27840.115516275595942No Hit
GCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTCCATAGGGCTGGC27750.11514283936017926No Hit
GAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGT27660.11476940312441651No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCC27600.11452044563390802No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA27260.11310968652102653No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAA27260.11310968652102653No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT27010.1120723636439078No Hit
GGGCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAAC25220.10464513184373768No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG25040.1038982593722122No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC24970.10360780896661896No Hit
ATCCAGTGAGTCCTGCAAGACTTCAGGCTCTACTACCTCCAGCAGCTCCC24580.10198958527831373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)