FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1975242
Sequences flagged as poor quality0
Sequence length126
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTACTTTTTCCTCCTTTTCCAGGCTCAGCAGGGAGCTGCTGGAGGTAG55920.28310455123979744No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA48650.24629893451030305No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA43500.22022617988074372No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC35930.18190176191069246No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC33960.1719283004310358No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA32500.16453680106032578No Hit
GCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTT32180.1629167464037318No Hit
CTTTACTTTTTCCCACTTTTCCAGGCTCAGCAGGGAGCTGCTGGATGAGA30410.15395581908444636No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT29770.1507157097712584No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC25000.12656677004640446No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG24770.12540235576197753No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC24570.12438982160160629No Hit
ATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGC23790.12044093837615846No Hit
GATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAG23020.11654268185872921No Hit
CAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGG22410.11345445266959693No Hit
CTTTACTTTTTCCTACTTTTCCAGGCTCAGCAGGGAGCTGCTGGATGAGA21590.10930306261207488No Hit
GGTTGTCTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTA21560.10915118248801918No Hit
GCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAGGACTCACTG20860.10560731292671985No Hit
CTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTC20840.10550605951068273No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)