FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792175
Sequences flagged as poor quality0
Sequence length126
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN529051.8947594617099575No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277510.9938846956225881No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN181580.6503174048904528No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120010.42980830356263483No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN72500.2596542122180737No Hit
CCCAGCTTCACTTCTTTGATCACCACCACGGAGACCACCTCACACAGTAC47910.17158666630852293No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC44310.15869349163286683No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC42930.15375110800719868No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC42080.1507068862087799No Hit
CTTCTTTGATCACCACCACGGAGACCACCTCACACAGTACTCCCAGCTTC41350.14809243689954962No Hit
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG39220.14046397521645312No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT36460.13057920796511682No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA35640.12764242928899514No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC35400.12678288431061807No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC34150.12230608754823749No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG31770.11378226651266486No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT31760.11374645213856581No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA30790.11027245785095849No Hit
CCTCACACAATACTCCCAGCTTCACTTCTTCGATCACCACCACCGAGACC28290.10131886432619731No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)