FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062903
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGA133270.4351100900028502No Hit
CTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGGC90850.2966140292395809No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG88710.28962719354808164No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG83760.27346605491587556No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG80970.2643570495049958No Hit
GTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCT76740.2505466219465651No Hit
CCTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTACTGTCGA76690.25038337812199735No Hit
GTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTC74520.24329859613575747No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT73420.23970723199526725No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG67300.21972618786817605No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA64730.21133545528539427No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA63410.207025818316806No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA62410.20376094182545124No Hit
CCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCCC61410.20049606533409642No Hit
CCCTGGCCAAACCGGGCATTTCCTGGGGCTGACGTGGCCCAGCCACAGCC58540.19112586980390825No Hit
GTCTTCATGATGGCCACAGTTGTGGGAGAGCCAGCCATTGTGGGGGCAGC57610.1880895346669483No Hit
GTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAG56750.1852817408843832No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT52150.17026330902415127No Hit
CCTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCAC49880.16285203938877596No Hit
GGCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTC48180.15730174935347285No Hit
CTCGGTATAGGACCTCCACTCGGCCCTGACACCTGTCACCTCCATTCACC46760.15266562473574904No Hit
CCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCA44830.14636441310743434No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT43840.14313218538099312No Hit
GGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCAT42160.1376471928755171No Hit
CACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCAC41320.13490469662277912No Hit
GTCGGCTGGGACTGGGAAGCTGAGCAGATGACACCAGCGTCTTCATGATG41030.13395788244028622No Hit
CCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCGGTATAGGACCTCC40550.13239074172443593No Hit
GGCCAAGTGTCTGGGCTGGGTGTCGGCTGGGACTGGGAAGCTGAGCAGAT40440.1320316053103869No Hit
CCTCCATTCACCAGTCTCAGGGCCAAAGTGGATTCAGATCCTGCTGTTGA39970.13049711335945016No Hit
CTCCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG39190.12795050969619345No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT38850.12684045168913283No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG37620.12282465360476645No Hit
GGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCAT36380.11877620675548656No Hit
GAGGGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATC35830.11698052468524142No Hit
CTCCATTCACCAGCCTCAGGGCCAAACTGGATTCAGATCCTGCTGTTGAT35440.11570722285361307No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC33760.11022223034813704No Hit
CCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTTCTCCCACGTCAAGA33060.1079368168041887No Hit
GGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGCTGAG32990.10770827544979387No Hit
GGCCCTGACACCTGTCACCTCCATTCACCAGCCTCAGGGCCAAACTGGAT32570.10633702732342487No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT32340.10558610573041327No Hit
GTCCTGAGCAGCGCACATCATCCAGGACAATGGGTCCTGAACCCTGGCCA32230.10522696931636423No Hit
CTCCAGATGTCAACACAGAAGTAGCTGTTCACAATTGCTCAGTTACCTGG32140.10493313043214232No Hit
CCTGCTGTTGATGCATGTGAGGTTGGCCAAGTGTCTGGGCTGGGTGTCGG31910.10418220883913072No Hit
GCATCATTGGTGTCCCAGTAGTCATCACACACGGTGCCCCAGGAGCCTCG31880.10408426254439009No Hit
CTGCAAACCACATTGGCATCATTGGTGTCCCAGTAGTCATCACACACGGT31430.10261506812328043No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)