FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3062903
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTC105830.3455218790800753No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT77100.2517219774834528No Hit
CCCAGCCCAGACACTTGGCCAACCTCACATGCATCAACAGCAGGATCTGA74820.2442780590831639No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA73680.2405560998830195No Hit
CGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTC70490.23014114387559775No Hit
CCAGGAAATGCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGA58140.18981991920736635No Hit
CATGAGTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAA46300.15116378154972587No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC45550.14871512418120977No Hit
CTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCA44950.14675619828639694No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC44720.14600527669338534No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT43800.14300159032133894No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT43530.14212007366867313No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43380.14163034219496995No Hit
CAGCAGGATCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGAC42840.13986730888963836No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC42580.13901844100188612No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT40490.13219484913495463No Hit
CCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATG40490.13219484913495463No Hit
GTCCTATCTGTGGAGCTGCCCCCACAATGGCTGGCTCTCCCACAACTGTG39530.12906056770325408No Hit
TCTGAATCCAGTTTGGCCCTGAGGCTGGTGAATGGAGGTGACAGGTGTCA39400.12863613375937796No Hit
GGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCCTATACCGAG39340.12844024116989666No Hit
CCTGAGGCTGGTGAATGGAGGTGACAGGTGTCAGGGCCGAGTGGAGGTCC39310.12834229487515603No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT38630.12612217886103477No Hit
GCCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTG38590.1259915838013806No Hit
CATCTGCTCAGCTTCCCAGTCCCAGCCGACACCCAGCCCAGACACTTGGC38350.12520801344345545No Hit
GACCCATTGTCCTGGATGATGTGCGCTGCTCAGGACATGAGTCCTATCTG38190.12468563320483868No Hit
GGCTGGGCCACGTCAGCCCCAGGAAATGCCCGGTTTGGCCAGGGTTCAGG37630.12285730236968001No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCTACCCTGCACCTGGTGCTC37100.12112691782926199No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC36630.11959242587832523No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC34790.11358505313423246No Hit
CGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCA34520.11270353648156668No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC34480.11257294142191247No Hit
CCCGGTTTGGCCAGGGTTCAGGACCCATTGTCCTGGATGATGTGCGCTGC33650.10986309393408802No Hit
CACTTGGCCAACCTCACATGCATCAACAGCAGGATCTGAATCCAGTTTGG33330.10881833345685449No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC33290.1086877383972003No Hit
CATGAAGACGCTGGTGTCATCTGCTCAGCTTCCCAGTCCCAGCCGACACC33220.10845919704280547No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC32020.10454134525317975No Hit
CGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACC31860.10401896501456298No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)