FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002300614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6590810
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG197530.29970519556776787No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA179430.2722427137180407No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA173740.2636094804735685No Hit
GTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCC126350.19170633048138241No Hit
AGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGAC122000.18510623125230433No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG112100.17008531576543703No Hit
GTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGT111230.16876529591962142No Hit
GCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACC88190.13380752896836656No Hit
GGCAGAGTTTGTGCTGGTTGTAGGTGCAGAGGTTATGCTGGTGGTGGGAA88120.1337013204750251No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTGT88020.1335495940559658No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC83220.12626672594112104No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGTTGT81960.12435497306097429No Hit
CTTGGATACAGAAAGATGGCTTGTGCTGGTCTTTGAAACAGGGGTTGTGC76030.11535759641075982No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTTCTGGTTGTAGGAG75250.11417413034209756No Hit
GCTGGTTGTAGGGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTTC75250.11417413034209756No Hit
GCTGGTTGTAGTAGCTGAGGTTGTGCTGCTTGTAGGGGCAGAGGTTGTGC71660.10872715189786991No Hit
GGCAGAGGTTGTGCTGGTTGTAGGGGCAGAGGTTGTGCTGGTTGTAGGAG70090.10634504711863944No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT68990.10467605650898752No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)