FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002300633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189311
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN978111.5803212990912883No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT208620.337064981869549No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC161230.2604974931781583No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT150800.24364585977340614No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC83540.13497463611054605No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG73430.11864002309788602No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT68510.11069083456946985No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA67690.10936596981473382No Hit
GCCAGCACAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCAC62090.10031811295312192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)