FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2716332
Sequences flagged as poor quality0
Sequence length126
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT52480.19320171466521766No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA45820.16868335682088936No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT44120.16242491713089563No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC40760.15005529515537866No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC36610.13477733944157047No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC34010.12520560815099185No Hit
CACCCCTCTTCCTGTCACCAGCCCTTCCTCAGCATCCACAGGTGACACCA33090.12181868784817172No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC31970.1176954805229994No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC28440.10470001457848303No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG27330.10061362160442833No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)