FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002300668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5617928
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATGCTGAGGAAGGGC130070.23152664113886828No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTGGTGACAGGAAGAGGGGTGGT114630.204043198844841No Hit
GTCGGTGACAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT105050.18699064850955727No Hit
GGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGGGTGG98440.17522474478135No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGGTGG95730.17040090225435428No Hit
GTGGATACTGAGGAAAGGCTGGTGACAGGAAGAGGGGTGGCCTGACCTGT84730.15082072963555246No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG83340.14834650782281297No Hit
GGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGGGGTGG80990.14416347094515986No Hit
CAGGAAGAGAGGTGGCGTGACCTGTGGATGCTGAGGAAGTGTCGGTGACA74780.13310957349399993No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGAGGATGCTGAGGAAGGGC74390.13241536737387877No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAAGGCTGGTGACAGGAAGAGGG66740.11879824732534841No Hit
GTCGGTGACAAGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGGGC64990.11568321986326631No Hit
GGTGGCGTGACCTGTGGACACTGAGGAAGCGTCGGTGACAGGAAGAGAGG64460.1147398115461786No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTCACCTGTGGATACTGAGGAAGCGT64400.11463301060462149No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG63690.11336919946286247No Hit
GGGGTGGTGTCACCTGTGGATGCTGAGGAAAGGCTGGTGACAGGAAGAGG62390.11105517906245863No Hit
GGGTGGTGTCACCTGTGGATGCTGAGGAAGTGTCGGTGACAGGAAGAGGG62260.11082377702241823No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT62110.11055677466852548No Hit
GTCGGTGACAGGAAGAGGGGTGGCGTGACCTGTGGATGCTGAGGAAGTGT61540.10954216572373301No Hit
GTGTGACCTGAGGATGCTGAGGAAGGGCTAGTGACAGGAAGAGGCGTGGT58150.10350791252575683No Hit
GTCGGTGACAGGAAGAGGGGTGGCCTGACCTGTGGATGCTGAGGAAGCGT57180.10178129730391704No Hit
GTCGGTGACAAGAAGAGAGGTGGCGTGACCTGTGGATACTGAGGAAGCGT57020.10149649479309811No Hit
GTCGGTGACAGGAAGAGGGGTGGTGTGACCTGTAGATGCTGAGGAAGGGC56320.10025048380826525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)