FastQCFastQC Report
Fri 31 May 2024
EGAF00002300669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5617928
Total Bases707.8 Mbp
Sequences flagged as poor quality0
Sequence length126
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACG142810.254204041062826No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC113670.20233438377992738No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC109330.19460911567396377No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC96480.17173591402381805No Hit
CACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCCA91420.1627290346191692No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT74570.13273577019855007No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGTATCC73110.13013694728732728No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC71070.12650571527438587No Hit
CCGACACTTCCTCAGCATCCACAGGTCAGGCCACCCCTCTTCCTGTCACC70410.12533090491725773No Hit
CCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCC69970.12454769801250568No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN68720.12232267839673275No Hit
CACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG66790.11888724810997933No Hit
CACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCA66020.11751663602666321No Hit
GCCCTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACCGAC61990.11034317278541127No Hit
CGCCACCTCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACG61980.11032537262848509No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC60590.10785115081574559No Hit
CAGCATCCTCAGGTCACACCACCCCTCTTCCTGTCACCGACGCTTCCTCA57870.10300950813182369No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA57140.1017100966762123No Hit
CAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACTA56590.1007310880452722No Hit
GGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGG56580.10071328788834602No Hit

[FAIL]Adapter Content

Adapter graph