FastQCFastQC Report
Fri 31 May 2024
EGAF00002300676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5342325
Total Bases658.6 Mbp
Sequences flagged as poor quality0
Sequence length101-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA172210.32235028756206335No Hit
GTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAG134660.25206253831431075No Hit
CCCACGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGA117160.21930526502973893No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG109380.20474231724951214No Hit
GCTCGAACAACGGCTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCC106230.19884600805828925No Hit
GTCCACTTTGTTTCCAGAACCTTGTTCTGCTGCTCCAGGAACCGCACCTT105530.19753571712690637No Hit
GGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTTCAAGACAACCTGAAG103120.1930245726345739No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT99230.1857430987444605No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT96160.17999653708825278No Hit
GTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAGGAACTTC90200.16884034572962145No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG87500.16378636642285896No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG86080.16112834767633943No Hit
CTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCC82850.15508229095010131No Hit
CTCCAATACATAAAAGGCACTTCTGTAGGGCTGGCATGAGTCAGTCAGTT80710.1510765443884451No Hit
GTCTGCCTTGGCTTGCAGTTCAACCTTGTTCATGTAGGCAGCATCCACAT79830.14942932150327806No Hit
GTCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT78220.14641565236109746No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC68850.1288764723224439No Hit
GCCTTGACCTCAGCGATGATGCTGTCCAGGTCCAGGTTGCGGTTGTTGTC68500.12822132685675244No Hit
GTCAAGAGCCAAGCCAAGGTACTGTTCCTCCAATGAGTAAACAGCACTGC66850.12513278394706426No Hit
GTACTTTTCAATTTCATCCATGTCAACAGCCAAGCCAACACGCTGTTGCT64010.11981674645402518No Hit
TACTGCTCGAACAACGGCTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTG61500.11511841754292373No Hit
GCTGGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACAT59340.11107523409751373No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG59130.11068214681809886No Hit
GTGCCCTGCTCCTGCAGCAGGGTCCACTTTGTTTCCAGAACCTTGTTCTG57420.10748129325714928No Hit
CTCCAATGCATAAAAGGAACTTCCATAGGGCTGGCAGGAGTCAGGCTGTT57370.10738770104776478No Hit
GTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTGCTGAGCC54940.10283911967167852No Hit
GTCCAGCTGCCTCCTGAGGTTGTTGATGTACTGCTCGAACAACGGCTCCA54630.10225884797349469No Hit
GGCTGGCCTAAGTCAGGCAGTTCAAGATAACCTGAAGGAGTCGAATAACA54610.1022214110897409No Hit
CCAGTGAGTCCTGCAAGACTTCAGGCTCTTTCTCATCCAGCAGCTCCCTG54240.10152882874029565No Hit
GGCAGGAGTCAGGCTGTTCAAGACAACTGGAAGGAGTTGAATAACATCTA53930.10094855704211182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)