FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5103413
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA303890.5954642510806004No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT171440.3359320517465469No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT148910.29178512497420844No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT139830.27399311010102456No Hit
GCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCG139710.273757973340586No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC139600.27354243131018396No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA107050.20976158504122633No Hit
GTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAAT106940.20954604301082433No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT106410.20850752231888736No Hit
GATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTC106380.20844873812877773No Hit
GCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCG104410.20458857631157815No Hit
GCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCC101700.19927840447167416No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC101020.19794596282918903No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC99300.1945756692629031No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT98840.19367431168122196No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC92930.1820938262296232No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT89360.17509850760657622No Hit
GCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCC77960.15276051536491364No Hit
GATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACC69750.13667324200490927No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC68530.13428268494045065No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC68360.13394957452982936No Hit
AGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC67650.13255834869723457No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC66660.1306184704236165No Hit
AGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACA62940.12332923085002134No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA62640.12274138894892497No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA61000.1195278532229314No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC60350.1182541957705559No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC58010.11366902894200409No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG55660.10906426738341576No Hit
GGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGC53320.10447910055486397No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT53310.10445950582482742No Hit
GCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTC53060.10396963757391377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)