FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002300691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2053813
Sequences flagged as poor quality0
Sequence length101-126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA52330.25479437514515685No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC44400.21618326498079427No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT36880.17956844172278585No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC36880.17956844172278585No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT32850.15994640213106062No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCCCTCTTCCTGTCACC32000.15580775854471657No Hit
CCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCC31340.15259422352473181No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC30530.14865033963656868No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC30340.1477252310702094No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT30150.14680012250385016No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA28610.13930187412388567No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC28130.13696475774571493No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC28080.1367213081229888No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT27440.13360515295209446No Hit
GTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCT26940.13117065672483327No Hit
CCGACACTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACC26870.1308298272530167No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC26720.13009947838483835No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC25770.1254739355530421No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG25640.12484096653395417No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC23960.11666105921035654No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC23440.11412918313400489No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACCCCTCTTCCTGTCACC23220.11305800479400997No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG22910.11154861713310804No Hit
CCACAGGTGACACCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCC22450.10930888060402774No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT22320.1086759115849398No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC21750.10590058588586206No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT21720.10575451611222637No Hit
CGTCAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAA21300.10370953928132698No Hit
CCCTGTCTGACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTC21040.10244360124315115No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC20950.10200539192224414No Hit
CCATCACTCTCGAAGTGGAGCCGAGTGACACCATTGAGAATGTCAAGGCA20600.1003012445631613No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)