FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002300699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002300699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385460
Sequences flagged as poor quality0
Sequence length101-126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC92020.27180944391604095No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC70990.20969085441860189No Hit
CCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCCACAGGTCACGCC67210.19852545887412643No Hit
CCACCCCTCTTCCTGTCACCGACACTTCCTCAGCATCCACAGGTCACGCC59820.1766968152038423No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA55240.16316837298328735No Hit
GGTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTA55240.16316837298328735No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA50360.14875378825920257No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT47400.14001051555770855No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC47350.1398628251404536No Hit
CACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGAC43300.12789990134280127No Hit
GATGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAG42950.1268660684220165No Hit
CACTGGATAGATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGAC42450.12538916424946683No Hit
CTTCCTCAGCATCCACAGGTCACGCCACCTCTCTTCCTGTCACCGACACT39470.11658681538107081No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA39210.11581882521134498No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC38890.11487360654091319No Hit
GCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCA37380.11041335593981319No Hit
CCGACACTTCCTCAGCATCCACAGGTGACACCACCCCTCTTCCTGTCACC35880.1059826434221642No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT35750.10559864833730129No Hit
GTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCCC35610.10518511516898738No Hit
CCAGCCTTTCCTCAGTATCCACAGGTGACACCACGCCTCTTCCTGTCACT35600.10515557708553637No Hit
TCCACAGGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATC34930.10317652549431983No Hit
CCACAGGTGACACCACGCCTCTTCCTGTCACTAGCCCTTCCTCAGCATCC34230.1011088596527503No Hit
GGTCAGGCCACCCCTCTTCCTGTCACCAGCCTTTCCTCAGTATCCACAGG34000.10042948373337743No Hit
AATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCA33950.10028179331612247No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)