FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002301154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42959809
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG12382542.8823545281590985Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1628860.37915904141938805Illumina Single End PCR Primer 1 (100% over 50bp)
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1045220.2433018265979721No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT932720.21711455933149051No Hit
AGAGCGTGTGCGGCTCCAGCCCAGCCCGCTGCTCACTTTCCACCCTCTCT776620.18077827115106587No Hit
GGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAACA585810.1363623381100228No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA547120.1273562459274435No Hit
GTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATCTGATACTGG525300.12227707995629124No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA431730.10049625686184964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)