FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002301161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93229430
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1457400.15632402772386358TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGTTCAGTTTGGGTTGCTTGTCTGTTTCCGGGTTGGGGGGAAAGTT1305070.13998476661286033No Hit
GGGGAAAGTTGGTTGGGTGGGAGGGAGCCAGGTTGGGATGGAGGGAGTTT1284860.13781699619959062No Hit
GGGATGTCTTCGTCTTGGCCCTCGACTTGGCCTTCCTCTTGGCCGTGCGT1139680.12224466029664667No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA968470.10388028758729943No Hit
GTCAGGGTTTAGCCATGTGGCCACAGCATCTGGGTATTTGTTGTAAAGCT956370.10258241415827599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)