FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002301169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16556723
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5545963.349672516717227TruSeq Adapter, Index 1 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2781371.6799036862548224No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC1536030.9277379346142349No Hit
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA1222090.7381231177208195No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1167450.7051214180487286TruSeq Adapter, Index 1 (100% over 50bp)
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA839560.5070810208034525No Hit
CCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCG782820.47281095419667285No Hit
CCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA761730.4600729262668706No Hit
CCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGA754730.45584503648457486No Hit
GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA704830.4257062221793528No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC650540.3929159169963767No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC598530.3615026959139197No Hit
CCCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGT576160.34799156813821186No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT572670.34588366308961016No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT539840.3260548600106434No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA490630.2963327948411047No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT470200.28399339651934746No Hit
CTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAG449050.27121912953426836No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC447220.27011383834832536No Hit
CCCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGT424060.25612556301147277No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA382490.2310179375471825No Hit
GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA377010.22770810383189957No Hit
CCCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTC363300.21942747970114615No Hit
CCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAA359500.21713233953361424No Hit
CTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGACTA348500.21048851273286384No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG344130.2078491015402021No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGT326720.19733373566737816No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA318760.19252602100065333No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGC300280.1813643919753927No Hit
GCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAATTGCG298170.18008998519815786No Hit
CGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGTCG297580.1797336344879358No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA294350.17778276534553367No Hit
GGGGGGGTCACTTATCCTGGGCTTCATCCAATGGTGGGTGCTAACTCTTT262890.15878142069538762No Hit
GGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCG256890.1551575151677056No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT256470.15490384178076785No Hit
GCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT256120.15469244729165307No Hit
GGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGG255070.1540582638243087No Hit
GACCTGCTTTCGCACCTGCTCGCGCCGTCACGCTCGCAGTCAAGCTGGCT250200.15111686050434014No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC249770.15085714727485625No Hit
CCCGTCAATTCATTTGAGTTTTAACCTTGCGGCCGTACTCCCCAGGCGGT241380.14578971937864757No Hit
CACCGGTATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATT239270.14451531260141273No Hit
CCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGG230770.13938144643719652No Hit
GTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAG229470.13859626690619875No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC228960.13828823493634582No Hit
CTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGAT225740.13634340563648978No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG224720.13572734169678383No Hit
CCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCCTTCG223230.13482740515740946No Hit
CTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAAC222030.13410262405187307No Hit
CCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC221190.13359527727799758No Hit
CCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATCGAC219860.1327919782193614No Hit
CTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTGAGC216740.13090754734496676No Hit
GTACGATTTGATGTTACCTGATGCTTAGAGGCTTTTCCTGGAAGCAGGGC216060.13049683805182946No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC213980.12924055080223304TruSeq Adapter, Index 1 (98% over 50bp)
CTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGT211440.1277064307955143No Hit
GGTCGGTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCA208150.12571932259783533No Hit
CCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAA207040.12504890007521416No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT204700.12363557691941816No Hit
GTCGGAAGATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTA200560.12113508210531759No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA192600.11632736743859277No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT190140.11484156617224314No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATGGTGAAATGGATT189230.1142919405005447No Hit
GCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGGGGCCGCC188610.11391747026268423No Hit
GGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTAT187990.11354300002482376No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTG187920.1135007211270008No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC184330.11133241765293772No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG183380.11075863261105474No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT183240.11067407481540883No Hit
GGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCGATGGGAAAC178310.10769643244016344No Hit
AGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCA174830.10559456723410786No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC173540.10481542754565622No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA171800.10376449494262845No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTC171710.10371013635971321No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG170600.10303971383709204No Hit
CCCAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTG167550.10119756186052035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGC167260.10102240642668238TruSeq Adapter, Index 1 (98% over 50bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC167070.10090764941830578No Hit
GGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG166040.1002855456360537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)