FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002301173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133129429
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG15637461.1746058041006096TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT9581890.7197424395172611TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTA2309530.17348005000457112TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT1655470.12435041691645804No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)