FastQCFastQC Report
Mon 6 Mar 2023
EGAF00002301174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133129429
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT17076131.2826713167980313Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG10148190.7622799914510262Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2437490.1830917490076518Illumina Single End PCR Primer 1 (100% over 50bp)
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT1396600.10490543003831257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)