FastQCFastQC Report
Sat 13 May 2023
EGAF00002301181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54481054
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG32726826.00700933575918TruSeq Adapter, Index 25 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT7175341.317033991302738TruSeq Adapter, Index 25 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATAACTCGTATG903930.1659163936145582TruSeq Adapter, Index 25 (97% over 40bp)
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT822280.15092953231044318No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC659440.1210402427236448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)