FastQCFastQC Report
Thu 1 Jun 2023
EGAF00002301191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93069174
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC25266622.7148215584249193TruSeq Adapter, Index 11 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC7045600.7570283153044852TruSeq Adapter, Index 11 (100% over 50bp)
GGGATGTCTTCGTCTTGGCCCTCGACTTGGCCTTCCTCTTGGCCGTGCGT2839520.30509779747266264No Hit
GTCACAGATCACGTCATCGCACAACACCTTGCCGTTGTCGCAGACGCAGA1694970.1821193771419955No Hit
GTCTCGTCACAGATCACGTCATCGCACAACACCTTGCCGTTGTCGCAGAC1694890.18211078138503733No Hit
GTCGCAGACGCAGATCCGGCAGGGCTCGGGTTTCCACACGTCTCGGTCAT1274650.13695727008386258No Hit
GGCAGTTCTTGGTCTCGTCACAGATCACGTCATCGCACAACACCTTGCCG1031980.11088311582092693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)