FastQCFastQC Report
Thu 23 Mar 2023
EGAF00002301192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93069174
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG26438312.84071608930364Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT7404060.7955437532947267Illumina Single End PCR Primer 1 (100% over 50bp)
GTTTCTCCTCGGGGTCGGAGCAGGAGGCACGCGGAGTGTGAGGCCACGCA2301380.24727628935441076No Hit
GCAGGAGGCACGCGGAGTGTGAGGCCACGCATGAGCGGACGCTAACCCCC2099140.22554621576420136No Hit
AGCAGGAGGCACGCGGAGTGTGAGGCCACGCATGAGCGGACGCTAACCCC1527030.16407473434759395No Hit
GAGCAGGAGGCACGCGGAGTGTGAGGCCACGCATGAGCGGACGCTAACCC1314510.141240105988262No Hit
GGAGTTTCTCCTCGGGGTCGGAGCAGGAGGCACGCGGAGTGTGAGGCCAC1009490.10846663364606632No Hit
CAGGAGGCACGCGGAGTGTGAGGCCACGCATGAGCGGACGCTAACCCCCT978540.10514115017288107No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)