FastQCFastQC Report
Wed 29 Mar 2023
EGAF00002301201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002301201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97022739
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT11900451.2265629812821508TruSeq Adapter, Index 20 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG9453200.9743282963800889TruSeq Adapter, Index 20 (97% over 43bp)
CCTCCATCAGGCAGTTTCCCAGACATTACTCACCCGTCCGCCACTCGTCA1536160.15832989419109267No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC1438910.148306470713015No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1161800.11974512490314254No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1116920.11511940515305387No Hit
GTCCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCA1103110.11369602748485588No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1063440.10960729525477528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)