FastQCFastQC Report
Sat 13 May 2023
EGAF00002312049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002312049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31681725
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT5690051.796003847644028TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTA1119450.35334250265728906TruSeq Adapter, Index 5 (97% over 37bp)
CAAGCAGAATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACG336200.10611795917046815RNA PCR Primer, Index 40 (100% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)