FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002313192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002313192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19657
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCTCGTAT289914.747926947143512TruSeq Adapter, Index 7 (97% over 38bp)
ATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAGAGCACACGTC4082.0755964796255784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCGCGTAT540.27471129877397366TruSeq Adapter, Index 7 (97% over 38bp)
AATGCGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAGAGCACACGT540.27471129877397366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCACGTAT380.1933153583965TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGATTGCATCCCGTAT350.17805361957572366TruSeq Adapter, Index 7 (97% over 38bp)
CGGTAGTTTATCACAGTTAAATTGCTAAGATCGGAAGAGCACACGTCTGA260.13226840311339472Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)