FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002313193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002313193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19657
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG304015.465228671719997Illumina Single End PCR Primer 1 (100% over 50bp)
TAGCAATTTAACTGTGATAAACTACCGCATAGATCGGAAGAGCGTCGTGT4032.050160248257618No Hit
TAGCAATTTAACTGTGATAAACTACCGCATTAGATCGGAAGAGCGTCGTG510.2594495599531973No Hit
TAGCAATTTAACTGTGATAAACTACCGAGATCGGAAGAGCGTCGTGTAGG220.11191941801902631Illumina Single End PCR Primer 1 (100% over 23bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)