FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002318792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871253
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16439618.86891637675853No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC849929.755145749856815No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT712348.176040713776596No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC676817.76823723992916No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT553376.351427197381243No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC487775.598488613525578No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT436065.004975592623497No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC418184.799753917633569No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG414274.754876023382415No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT404994.648362760300396No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC350054.017776696321275No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC335423.8498576188546836No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT308763.5438615419401716No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC170831.9607393030497455No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTG151901.743466019629201No Hit
GCTCTGATAGGAAAATGAGATCTACTGCTGTGATGACTTGCCAT20880.23965484193454717No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA18770.21543684785016523No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG17040.19558038824543503No Hit
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGA10550.12108997042190958No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT9650.11076002033852395No Hit
TGTCTCAGTGTTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGTG9620.11041568866907775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)