FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104516
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN79955972.38998801284907No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT269532.4402543738614924No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC235902.135777118665551No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC189791.7183091960641586No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC188661.7080784705699146No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCA177401.6061333652024958No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC175661.5903798586892357No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG119431.0812880936084222No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT104170.943128030739256No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT104150.9429469559517472No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC93450.846071944634573No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGAC82110.7434025401171193No Hit
TAGGCAAGTCGAGGCAATGGGGCAGAGTTGTTTAGAAGC75480.6833762480579729No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT66810.6048803276729355No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC63570.5755462120965201No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT60380.5466647834888766No Hit
ACTCAAGAAGCAGAAAGGGAAGAGCACACGTCTGAACTCCAGTCACATTA29960.2712500316880878TruSeq Adapter, Index 27 (96% over 33bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC26320.2382944203614977No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC26150.23675528466767345No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC25360.22960283056107836No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA23480.21258180053525705No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC22670.20524827164115322No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA22670.20524827164115322No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC18010.16305784615161756No Hit
CTGTTTCGGTTGGCTTTGGAGTCACAGGTAAGTGC17950.16251462178909132No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG17670.159979574763969No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACACAG17430.1578066773138642No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT17220.15590539204502243No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG15070.1364398523878332No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA15010.13589662802530703No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA14670.13281835663765848No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT13620.12331193029344979No Hit
TGTCTCAGTGTTTGCCCCAATGTCAACAGGAATGCA13070.11833237363695953No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA12970.11742699969941586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)