FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468666
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT263195.615726338159798Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC250845.352212449804338No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC242885.1823686804675395No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT203794.348299215219368No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC193844.135994503548369No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG189404.041257526682115No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC183063.905979951607328No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT162013.456832797770694Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC161853.4534188526583964No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT158823.3887672670942637No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT136272.9076143778298404No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT116402.4836450691964No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA104092.2209846671190143Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA99502.123047116709981No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT96812.06565016450948No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC96802.0654367929399617No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC80611.7199882218893625No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT77551.6546965216166738No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA76721.6369866813466305Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC72841.5541985123734174No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC69481.4825056650151707No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC67411.4383377501248225No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA60351.287697422044697No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC59911.278309072985879No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC54181.1560471636517264No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG53951.1511396175527988No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA49161.0489346357533937Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT47331.0098876385314914No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC46020.9819359629245562No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG42520.9072559135930491Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC38170.8144392808524621No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG29310.6253920702589905Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT28560.6093892025450961No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT26880.5735427788659728No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA25990.5545527091788182No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG25350.5408969287296284Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT23720.5061173628980979No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC22800.48648717850238765Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG21280.45405469993556175No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA19860.42375593706392184No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC19390.4137274732965481No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC19360.4130873585879923TruSeq Adapter, Index 27 (97% over 39bp)
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT19130.40817981248906476No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC18770.40049843598639545Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC18750.4000716928473582No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT14530.3100288905105128No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA14420.30768180324580835No Hit
ATACCAGGGGCCATGCTGATCGGAAGAGCACACGTCTGAACTCCAGTCAC14030.2993603120345833Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC13910.2967998532003602TruSeq Adapter, Index 1 (97% over 38bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA13670.291678935531914No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA12320.2628737736469042No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA11580.24708427750252843No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA10830.23108140978863415No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC10760.229587808802004Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC10380.22147968916029753No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC10100.215505285213777No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC9800.20910413812821926No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG9480.20227624790362433No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG9400.2005692753474756No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA9220.196728587096141TruSeq Adapter, Index 1 (97% over 37bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC8740.1864867517592486No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT8720.1860600086202114No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC8520.18179257722983957Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT7490.15981530556942472Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CAGAGTCACAGGTAAGTGCTGATCGGAAGAGCACACGTCTGAACTCCAGT6920.147653126106865Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG6750.14402580942504897No Hit
GCCATTCAAACCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGA6740.14381243785553036Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT6540.13954500646515855No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG6090.12994328583682194No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT6060.1293031711282662No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG5950.12695608386356166No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG5390.11500727597052059No Hit
ACTCAGGACATGTCAGCCTCGATCGGAAGAGCACACGTCTGAACTCCAGT5210.11116658771918594Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA5140.1096729867325558No Hit
TCATCATTAGCGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG5010.10689915632881412No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC5010.10689915632881412No Hit
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC4740.10113812395181217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)