FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337759
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT213196.311896944270915No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC155314.5982490473977No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT154034.560352203790276Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT142544.220168818595507No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC113903.37222694287939No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC108043.1987304557391516No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC100222.967204426825044No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC92942.751666128807819No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA81502.4129630890664644Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA76082.2524936419162778No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT74102.193871961961043Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT73902.1879505801473833No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC70002.0724836347810123No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT68092.015934438460559No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC67071.985735391210893No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT66401.9658987621351316No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG65741.9463582021500538No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC61971.8347401549625622No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA56731.6795999514446693No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA55151.632821035116755No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA54291.6073590933180166No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC53751.5913713624211345No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC53531.5848578424261086No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT51551.5262361624708742No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG48831.4457053698050977No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT45201.3382322898871681No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG44091.3053686208213549No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA39481.1688807700164912Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG38321.134536755497263No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA37501.1102590900612566No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC37271.1034495009755476No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC36881.0919028064389107No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT36571.0827246646277375No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC36241.0729543846351985No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA33981.00604277014084No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG33260.9847257956116638No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT32620.9657773738079519No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC32210.9536385410899487No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA28280.837283388451529No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA27890.825736693914892Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA24350.7209282358131094No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG23910.7079011958230572Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA22790.674741457666561No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC18770.5557216832119943No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG16960.502133177798371Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT15300.4529857087449928No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC13920.4121281742307385No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC11830.3502497342779911No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC10630.31472144339603086No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC10230.3028786797687108No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC9830.29103591614139074TruSeq Adapter, Index 1 (97% over 38bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC9680.28659487978114573Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT9290.2750481852445087No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG9240.27356783979109367No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9220.2729757016097277No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG8850.2620211452544566No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC8250.24425699981347648Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG7900.23389458163957144No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA7730.22886140709796038TruSeq Adapter, Index 1 (97% over 37bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC7230.2140579525638103Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC6570.19451739257873218No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTC6530.19333311621600016TruSeq Adapter, Index 27 (97% over 39bp)
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC6340.18770780349302313Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC6220.18415497440482712No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC5900.17468076350297107Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC5740.16994365805204303No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG5660.16757510532657902Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GGATTCGAGATGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT5300.15691661806199095No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT5270.15602841078994192No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC5130.1518834435203799No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG4850.14359350898125586Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC4830.14300137079988987No Hit
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG4570.1353035744421318No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT4150.12286867263344572Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT4070.12050011990798173No Hit
TAGGCAAGTCGAGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGT3760.11132197809680867No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT3710.10984163264339365No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA3630.10747307991792963Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATCTCCTACCACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT3520.10421631992041662No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)