FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350515
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC185935.304480550047787No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC158074.509650086301585No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT151904.33362338273683No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC141044.0237935609032425No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC118723.387016247521504No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC115663.2997161319772337No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT108943.1079982311741294Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT108653.099724690812091No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT102372.920559747799666No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG101092.884042052408599No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG100682.8723449781036474No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA96682.7582271800065614No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC95572.7265594910346205No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT91592.61301228192802No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT76862.192773490435502No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA69941.9953496997275437No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA64001.8258847695533715Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC63271.8050582714006533No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT62081.7711082264667704No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC60821.7351611200661883No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC55321.5782491476826956No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA55161.5736844357588118No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA55151.5733991412635693No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC54961.5679785458539577No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT52631.5015049284624054No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT46531.3274752863643495No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA46481.3260488138881361No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG46281.3203429239832818No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA43551.2424575267820208No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC42491.212216310286293No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC41441.182260388285808No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA39051.1140750039227993No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA38451.0969573342082364No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC38201.0898249718271686No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT37821.0789837810079455No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG37231.0621514057886254No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA35020.9991013223399854No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC30090.8584511361853273No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG26010.7420509821262999No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT24620.7023950472875626No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT22660.6464773262199905Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT22180.6327831904483403No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG21420.611100808809894No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA19480.5557536767328074Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT18240.5203771593227108No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG16270.4641741437598962Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA14150.40369171076844074Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG13600.3880005135300914No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC10330.2947092135857239No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC9770.2787327218521319No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT8940.2550532787469866No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC8370.23879149251815188No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC8320.2373650200419383No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC8120.231659130137084No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT7040.20084732465087085No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT7010.19999144116514272No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC6990.1994208521746573No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA5610.16005021183116272Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG5350.15263255495485215Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC4880.13922371367844458Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC4870.13893841918320185No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC4420.1261001668972797TruSeq Adapter, Index 1 (97% over 38bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4300.12267663295436715Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG4100.11697074304951285No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC3840.1095530861732023No Hit
GGATTCGAGATGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT3760.10727073021126057No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)