FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356532
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC169424.751887628599957No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT146834.118283912804461No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC143904.036103351171844No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT141073.9567275868645733No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC135783.8083538083538087No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT133483.7438434698708676No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG129283.6260419822063654No Hit
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC123313.458595581883253No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC119643.355659520043082No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC107363.0112304084906825No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA96582.708873256818462No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA87722.4603682137928713No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT87282.4480271055613523No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC85152.388284922531498No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG84902.3812729292181345No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT79202.2213994816734544Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC75432.1156586225079375No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT70731.9838331482167098No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA69571.9512974992427046No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT68751.9282981611748735No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC68411.9187618502686996No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA67581.895482032468334No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT63971.7942288490233695No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG57741.6194899756543595No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC54511.528895022045707No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC52731.4789696296545611No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT47361.32835201328352No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA46911.3157304253194664No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC42601.1948436605970854No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA40631.1395891532877833Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA37441.0501161186092693No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA36831.0330068549246632No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT36801.0321654157270594No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC35801.004117442473607No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG31470.8826697182861566No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA30640.859389900485791No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA29670.8321833664299418No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC28480.7988062782583331No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG22900.6422985875040669No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG21030.5898488775201104No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT19650.5511426744303456No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG18330.5141193497357881Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT17500.4908395319354223No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG16530.46363299787957324No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15170.42548775425487756Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC13860.3887449092928545No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC13370.3750014023986627No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT13220.37079420641064476Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA11610.3256369694725859Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG11570.3245150505424478No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT10910.30600338819516903No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9050.253834157943747No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC9010.2527122390136089Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC8380.23504201586393367No Hit
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC8060.2260666644228288No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC5830.16351968406762926No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC5640.15819056914947324No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT5150.14444706225528145No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT4810.13491075134910752No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA4710.13210595402376224No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTAC4690.1315449945586932TruSeq Adapter, Index 1 (97% over 38bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG4330.12144772418745023No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC4320.12116724445491568No Hit
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC4300.12060628498984664No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG4220.11836244712957042Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTA4110.11527717007169062TruSeq Adapter, Index 1 (97% over 37bp)
TGTGAAGATCCTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC3970.11135045381620724No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC3890.10910661595593103No Hit
CTGTTTCGGTCGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG3770.1057408591655167No Hit
ATCCAGACTGCGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT3670.10293606184017144No Hit
GGATTCGAGATGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT3610.10125318344496427No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)