FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349718
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC229736.569007028520121No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC152044.347502845149521No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT141944.05869872297108No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC130503.731578014285796No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC128193.665524794262806No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC119553.418468594696298No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT117343.35527482142755No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT116873.341835421682613Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG110283.1533978805780656No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT98242.80912049136733No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA92872.6555682006645354Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC91222.6083873292195423No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT87382.4985845738566503No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC67551.931556282490464No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA59421.69908326137059No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT58781.6807828021434414No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG57401.6413224369349018No Hit
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC56661.620162530953511No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC56111.6044355738051803No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG55411.5844194465254862Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA54831.5678346553508828No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA51921.484624754802441No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT50151.4340125472523577No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC49871.42600609634048No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT49611.4185715347794507No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA48471.385973841781092No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC45851.311056336819952No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA45311.2956153243470454No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA44301.2667349121292013No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA43641.2478625635512042No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC43011.2298480489994796No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC42981.228990214973207No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC41591.1892439050892434No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG40821.16722616508158No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA40321.15292893131037No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA39901.1409192549425538No Hit
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT38331.0960259409009545No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG35041.001950142686393No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT28420.8126547675555734No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG24860.7108584631045585No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG24230.692843948552834No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG23890.6831218295884113No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT20840.5959087035840306No Hit
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC17420.49811562458895453No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA16570.47381032717789767No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA15010.42920295781172263Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC12230.3497103380437953No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA11410.326262874659011Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT10940.3128234749140736No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC10520.3008137985462573No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC10400.2973824624411669No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC9320.2665004374953534Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC9010.25763615255720324No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9000.25735020788177904No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT8590.24562647618938688No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACTAAGTACTTCATACC7820.22360873618172358No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGG7270.2078817790333926TruSeq Adapter, Index 6 (97% over 36bp)
CATCTCCTACTACGAGCTGAATCCGGTGTGTTAAGGTGATGATCGGAAGA7270.2078817790333926No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC6970.19930343877066664No Hit
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC6910.19758777071812147No Hit
ATCCAGACTGTGTTTCTTCCTCTATTATTGGATCGGAAGAGCACACGTCT6230.17814353278927592Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCG5810.16613385642145956TruSeq Adapter, Index 6 (97% over 36bp)
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT5730.163846299018066No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC4810.1375393888790397No Hit
TCATCATTAGTGGATCAACAGGAATGCAGGATCGGAAGAGCACACGTCTG4510.12896104861631372Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4330.12381404445867815Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT4190.11981081900273936No Hit
TGTCTCAGTGCTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC3990.11409192549425536No Hit
CATCTCCTACCACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT3990.11409192549425536No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)