FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345837
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC253917.341898061803683No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC168004.857779821129608No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC134183.8798624785664924No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT130953.7864658784340604No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG124593.6025642137770104No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT117873.4082530209318262No Hit
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC111103.2124960602827346No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT97232.81144007147876No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA94192.723537389001177No Hit
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC92232.666863291087998No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT86122.490190465450487No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA85002.457805266642956No Hit
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT82202.376842269624129Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC74952.167205938057524No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC74462.1530374135792294No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG73142.114869143556068No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA72532.097230776348395No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC69101.9980511050003325No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT68011.9665333668751463No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT64301.859257395825201No Hit
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT63551.8375708787665863Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT58331.6866327200386309No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC50021.4463461110291842No Hit
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA49121.420322290558847No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA42721.2352640116586715Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT41221.1918909775414428Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC37821.0935787668757246No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA37481.083747545809153No Hit
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC35641.0305432906253524No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC35371.0227361444842513No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC33230.960857282477005No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA31730.9174842483597765No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC30590.8845207424306827No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT30230.8741112142425478No Hit
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA24340.70379976694223No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG24130.697727542165818No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA24060.6957034672403473No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA23370.6757518715464221No Hit
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC21170.6121380881744868No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG19790.5722348967866364Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA18310.5294401697909709Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC18310.5294401697909709No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT16520.477681682411078No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG16470.4762359146071704No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT16220.4690070755876323No Hit
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG15330.44327240867807666No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC15190.4392242588271354TruSeq Adapter, Index 2 (97% over 37bp)
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG14880.43026049844290803No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA13830.39989937456084806Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT13280.3839959287178642No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG12410.3588395689298716No Hit
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT11360.32847844504781154No Hit
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG10330.29869562828731455Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG10030.2900210214638688No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT9940.2874186394168351TruSeq Adapter, Index 2 (97% over 37bp)
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT9610.2778765719110448No Hit
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC9500.27469588274244805Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GAGAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGAC8180.23652761271928682No Hit
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC7870.2275638523350596No Hit
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC7360.21281702073520184No Hit
TGTGAAAGCATTGATGGAATGATCGGAAGAGCACACGTCTGAACTCCAGT7140.20645564239800832Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC6710.19402203928440276Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC5920.17117890798266236No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG5600.1619259940376536Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT5260.15209477297108176TruSeq Adapter, Index 2 (97% over 37bp)
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT5140.14862493024170345No Hit
GAAAAATCAATCTTCTGTTCAGGTGGAGGACCTTTTTTACAACATAGCCA4690.13561302000653488No Hit
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA4580.13243233083793812No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA4430.12809502742621523No Hit
GTGTGTCACCTTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC4150.11999872772433255No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG3890.11248073514401294No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA3750.10843258529307159Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC3740.10814343173229006No Hit
GGACCTGATTTCCTTACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT3570.10322782119900416No Hit
CAGAGTGGACCTAAGCAAGCTGATCGGAAGAGCACACGTCTGAACTCCAG3550.1026495140774411Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
TTGGAATATGAAGCAAGGCAAATCAGAGTTGCGATGGAAGAACAACTAGG3520.10178205339509652No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC3480.10062543915197043Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATCCAGACTGCGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT3470.10033628559118891No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)