FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002318822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002318822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333780
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGGGCCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT187635.621367367727245Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACTTACTACACCTC153254.59134759422374No Hit
CATCTCCTACTACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT136684.094912816825453No Hit
GTGTGTCACCCTCCTGAACCGCCTGGAAGGGGACCAGATCAGCAGCCCTC114843.4405896099227036No Hit
GGATTCGAGAAGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT109463.279405596500689No Hit
ACAAACTGGTGCAGGACCGATCGGAAGAGCACACGTCTGAACTCCAGTCA104503.130804721672958Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CACACCCCCAGGATTCTTACAGAAAACAAGTGGTTATAGATGGTGAAACC104183.121217568458266No Hit
ATCCAGACTGTGTTTCTCCCTTCTCAGGATTCCTACAGGAAGCAAGTAGT99572.9831026424591047No Hit
CCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCAGT95912.8734495775660616No Hit
TCATCATTAGTGGATCTACAGATCGGACACTCAAAGTGTGGAATGCAGAG92812.78057403079873No Hit
TGTCTCAGTGTTTGCCCTATTGTCGACTGCATCAAAATGGTTTCCAGGAC89792.6900952723350713No Hit
CTGTTTCGGTTGGCTTTGGTGAGATCCATTGACCTCAATTTTGTTTCAGG69872.0932949847204747No Hit
TGGCCATCTACAAGCAGTCACAGCACATGACGGAGGTTGTGAGGCGCTGC68462.0510515908682367No Hit
TAGGCAAGTCGAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGT68382.0486548025645637Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACACTAGAGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT67122.0109053867817126No Hit
GTGTAGAAATGGCCGGATTGAAGTTTGTAAATCCTTTTGGTCTTGCTAGC58631.756546228054407No Hit
CAGGTTATTGCGAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACAC58411.7499550602193061No Hit
GCTCTGATAGGAAAATGAGATCTACGATGGGAAGTTTGCGATCGGAAGAG50421.5105758283899575No Hit
TGGACAGCAGGAATGTGTTTCTCCATACAGGTCACGGGGAGCCAATGGTT48761.460842471088741No Hit
TGTGAAGATCTTGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGC45631.367068128707532No Hit
TGCATACATTCGAAAGACCCTAGCCTTAGATAAAACTGAGCAAGAGGCTT43791.311941997723051No Hit
ATCATCTTTGTCATCAGCTGAAGATGAAATAGGATGTAATCAGACGACAC43231.2951644795973394No Hit
CAGAGTAACAGACTAGCTAGAGACAATGAATTAAGGGAAAATGACAAAGA42651.2777877643957098No Hit
GACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACCAGA42501.2732937863263227No Hit
TGGCCATCTACAAGCAGTCTTTGTGTCAAGAAGAGATCGGAAGAGCACAC41341.238540355923063No Hit
AAGCCCCAGTGATCTTCCAGATAGCCCTGGACAAACCATGCCACCAAGCA40681.2187668524177604No Hit
TCTGCAGAGTTGTTAGCGGTTCTCGAGATGCCACTCTTAGGGTTTGGGAT40471.2124752831206185No Hit
ATACCAGGGGCCATGCTGATGGCCATCCGACTTCGGGGCATGGATCTGGA39011.1687338965785847No Hit
GGTGCTCAGACACCCAAAAGTCCACCTGAACACTATGTTCAGGAGACCCC37881.1348792617892023No Hit
GTGTAGAAATGGCCGGATTGAAGTTTATAAATCCTTTTGGTCTTGCTAGC37661.1282880939541016No Hit
TCATCATTAGTGGATCTACAGGAATGCAGGATCGGAAGAGCACACGTCTG36661.0983282401581882Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAGGTTATTGCGAGTGTTTTGAGGAATTTGTCTTGGCGAGCAGATGTAAA35631.067469590748397No Hit
CAGAGTAACAGACTAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA35511.0638744082928875Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAATTCACTTACACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC35011.0488944813949308No Hit
TTGGCTCTGACTGTACCACCATCCACTACAACTACATGTGTAACAGTTCC33210.9949667445622865No Hit
CTACTGAAGTGGGATGCCAGCCTCTGCCTTTTGCAACAACATTATGGGGA31050.9302534603631134No Hit
CAGAGTAACAGACAAGCATTCCACAGGGGATCGGAAGAGCACACGTCTGA27780.8322847384504765Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGTGTGAGACGTTGACTGAGTGCTGGGACCACGACCCAGAGGCCCGTCTC25830.7738630235484452No Hit
GGATTCGAGACGTGACAGGCTATGTCCTCGTGGCCATGAATGAATTCTCT25050.7504943375876326No Hit
TCTGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTCTGAACTCCA23000.68907663730601Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
GAGAGAACGCGGAATTGGTCTAGGCAACTACCATCCAGCAACAGAAAATC19200.5752291928815387No Hit
GGACCTGATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAG19090.5719336089639883No Hit
GACCTCGTCTTCCACACAATTAAACAGCATGCGATCGGAAGAGCACACGT18930.5671400323566421No Hit
ATAACACTAGCGACAGTTTGCCATGACCCCAAGCTCCCCTACCATGACTT18300.5482653244652166No Hit
TGTCTCAGTGTTTGCCCTATTGTCAACAGGAATGCAGGATCGGAAGAGCA18200.5452693390856252No Hit
TCATCATTAGTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTC15910.4766612738929834TruSeq Adapter, Index 2 (97% over 37bp)
GGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT15400.46138174845706753Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGGCAAGTCGAGGCCCAGTGGATCGGAAGAGCACACGTCTGAACTCCAG14670.43951105518605066Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
ACAAACTGGTGGTGGTTGGAGCAGGTGGTGTTGGGAAAAGCGCACTGACA13190.3951704715680988No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTGTCA13160.39427167595422136No Hit
TCCTCTATTATTGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCT12510.37479777098687755TruSeq Adapter, Index 2 (97% over 37bp)
TAGGCAAGTCGGGCAGAGTTGTTTAGAAGCCGATCGGAAGAGCACACGTC12080.3619150338546348No Hit
TAGGCAAGTCGAGGCAATGGAAAAGCTCATTAACTTAACTGACATTCTCA11600.34753430403259633No Hit
GACCTCGTCTTCTACACAATTAAACAGCATGCGATCGGAAGAGCACACGT11090.33225477859668046No Hit
GCCATTCAAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTC10980.32895919467912993Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGACAGCAGGAATGGTAAGTGGCGATCGGAAGAGCACACGTCTGAACTC10600.31757445023668285Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATTATAAGGCCTGCTGAAAATGACTGGTGCAGGACCGATCGGAAGAGCAC10070.3016957277248487No Hit
GTGTGTCACCCTCCTGCTGTGATGACTTGCCATTGATCGGAAGAGCACAC9710.29091018035831984No Hit
ACTCAAGAAGCAGAAAGGGAAGAATTTTTTGATGAAACAAGACGACTTTG8980.269039487087303No Hit
TAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCAT8340.24986518065791838TruSeq Adapter, Index 2 (97% over 37bp)
GGATCCATTTTGTGGATGGGATCGGAAGAGCACACGTCTGAACTCCAGTC7830.23458565522200253Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
ATCCAGACTGTGTTTCTCCAATGTCAACAGGAATGCAGGATCGGAAGAGC7770.2327880639942477No Hit
TTAACAGATGTTTTACTGTGCCTGGCTAACTATAGTCGAGGGGGTGATGG7010.21001857510935346No Hit
CCAAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTGAACTCCAG6970.20882018095751692Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAATTCACTTCCACCGGGCCCTCCAGCTCCTAGACGAAGTACTTCATACC6950.20822098388159865No Hit
GAGAGACAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCCAGTC6920.20732218826772125Illumina Multiplexing PCR Primer 2.01 (96% over 32bp)
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT6900.20672299119180296No Hit
ACTCAAGAAGCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC6850.20522499850200732Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
CCAAGGGAGTGAAAATCTGGGATGCCAATGGATCCCGAGACTTTTTGGAC6090.18245550961711307No Hit
TGGCCATCTACAAGCAGTGGAATGGTAAGTGGCGATCGGAAGAGCACACG5630.16867397687099286No Hit
CTGTTTCGGTGGACAAAGTCAACTTCCAGATCGGAAGAGCACACGTCTGA5350.1602852178081371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGAGAACGCGGAAGTGTGAAAGCATTGATGGAATGATCGGAAGAGCACA5290.1584876265803823No Hit
ATACCAGGGGTCATTGTCACAGAGGATAAGGATCGGAAGAGCACACGTCT5220.15639043681466833Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCAGACTGTGTTTCTCCCATGTCAACAGGAATGCAGGATCGGAAGAGC5060.1515968602073222No Hit
ACTCAAGAACCAGTAGGCAACCGGATCGGAAGAGCACACGTCTGAACTCC4920.14740248067589432Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATTATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAG4890.1465036850620169No Hit
GCCATTCAAACCAGGAAGATACCGGATGTAAATTTCGAGTTTTGCCTCAG4700.14081131284079332No Hit
TGGCCTCTGATTCCTCACTGAGATCGGAAGAGCACACGTCTGAACTCCAG4420.13242255377793755Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGGCAAGTCGAGGCAAGGTATTGATTGTATAGTGATCGGAAGAGCACAC4330.12972616693630534No Hit
TAGGCAAGTCGAGGTATTGATTGTATAGTGATCGGAAGAGCACACGTCTG3890.11654383126610342Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TAGGAGAGACATTTAAGGTTCCTTCAAGCTGCCCTATTGTTACTGTTGAT3880.1162442327281443No Hit
CATCTCCTACCACGAGCTGAACCAGCGCGTCGGGGAGACATTCCACGCCT3600.10785547366528853No Hit
GACCTCGTCTTCTACTTCTGGAGTCACAGGTAAGTGCTGATCGGAAGAGC3560.10665707951345198No Hit
GTGTAGAAATGGCCGGATTGATGACTTGCCATTGATCGGAAGAGCACACG3380.10126430583018754No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)